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FS significantly increased plasma CORT levels in direct decapitation and isoflurane groups, while this stress response ‘disappeared’ following i.p. sodium-pentobarbital injection. In control animals, both the injection of saline and pentobarbital caused a significant increase of plasma CORT. Neither the sex hormone levels nor the mRNA expression of stress-related molecules in the brain showed significant differences among the groups. testosterone.

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Hysterectomy is the most common major gynecologic operation, together with bilateral salpingo-oophorectomy in the majority of women over the age of 45. testosterone.

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Mean age (standard deviation) was 58.0 (9.2) years. Age and prevalence of CV risk factors was similar in men with TT ≤ 8 nmol/L or 8-12 nmol/L and significantly higher than in men with TT >12 nmol/L. Low T was therefore considered as TT ≤ 12 nmol/L, with a prevalence of 33.3%. Obesity, hypertension, hyperlipidemia, and severe ED were the variables most strongly associated with low T: obesity in middle-aged men; hyperlipidemia, and hypertension in older men. Severe ED was a risk factor in both groups. Hypolipidemic therapy had the greater effect in young men. Multivariate analysis showed that severe ED and obesity were the strongest predictors of low T. testosterone.

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We analyzed the expression of receptors and enzymes relevant to the action of androgens (AR, 5α-reductase 1 and 5α-reductase 2) and estrogens (ERα, ERβ, and aromatase) in male and female HUVECs. Furthermore, we analyzed the effect of testosterone (T), 17β-estradiol (E2), dihydrotestosterone (DHT), and several androgenic-anabolic steroids (AAS) on VCAM-1, ICAM-1, and E-selectin gene expression and on adhesion of U937 cells to TNF-α-stimulated male and female HUVECs. testosterone.

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This study examined the effect of an intensification period (IT; 4weeks; after a habitual training period; HT) followed by a tapering period (TP; 2weeks) on salivary hormones (testosterone – T and cortisol – C), well-being (WB), and physical performance in 23 rhythmic gymnasts (RG; Under-11 group [G1], Under-13 group [G2], and >13 group [G3]). The session-rating of perceived exertion was used to quantify the daily internal training load (ITL). The WB questionnaire was completed daily. Physical performance tests and saliva sampling were carried out at the beginning of the IT (T1), after IT (T2), and after TP (T3). A higher ITL was observed for IT compared to HT (2310±327 vs 2940±334, 2449±237 vs 3902±273, 2278±436 vs 3954±866 arbitrary units [UA], for G1, G2, and G3, respectively) and TP (vs 1781±260, 2305±298, 2415±522AU). No significant change was detected for T concentration (206±39, 221±35, 216±51 ρmol/L, for T1, T2, and T3, respectively [whole group]; p=0.16), C concentration (5.7±1.0, 5.8±0.8, 5.0±0.7nmol/L; p=0.07), and WB (19±3, 19±2, 19±2; p=0.44). A significant lower WB score was observed for the G3. Physical performance increased for sit-ups from T2 to T3 (ES=0.80), and T1 to T3 (ES=0.78) and for push-ups (T2-T3; ES=0.61; T1-T3; ES=0.55). In summary, a period of IT followed by TP, seems to be a useful approach to improve physical performance of youth RG, while maintaining the perception of WB and the hormonal milieu. testosterone.

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In a last few decades, anabolic-androgenic steroids (AASs) abuse has become serious health concern especially among adolescents. AASs abuse has been reported to be involved in pathogenesis of various mood disorders, including depression. In order to evaluate the effects of chronic (6 weeks) testosterone enanthate (TE) treatment in supraphysiological dose and exercise on depression-like behavior in rats, 32 male rats were divided into four groups: control (C), testosterone enanthate (T, 20 mg/kg/w, s.c.), exercise (E, swimming for 1 h/day), and combined group-testosterone enanthate plus exercise (T + E). TE produced prodepressant effect in tail suspension test (TST) parameters compared to the control and exercise groups, while exercise induced the opposite effect. Simultaneous TE administration along with exercise attenuated the antidepressant effect of exercise reversing the parameters of TST to the control values. Oxidative stress markers in rat hippocampus were significantly altered following applied protocols. TE administration increased index of lipid peroxidation (TBARS) and decreased superoxide dismutase activity (SOD), while exercise induced the opposite effect, with no change in glutathione (GSH) levels. Our results indicate that TE chronic treatment resulted in clear depressive-like behavior, even abolishing beneficial antidepressant effects of exercise in TST that was accompanied with increased oxidative damage in rat hippocampus. The antidepressant effect of exercise correlated with the improvement of redox status in hippocampal tissue. Behavioral parameters obtained in TST significantly correlated with the levels of oxidative stress markers. testosterone.

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